Pham Hong Hien2, Bui Huong Quynh1, Vu Thi Linh1, Le Quang Man3, Duong Thi Nguyen4 and Nguyen Van Giang1
1 Vietnam National University of Agriculture, Faculty of Biotechnology, Trau Quy, Gia Lam District, Ha Noi City, Vietnam
2 Vietnam Academy of Agricultural Sciences, Vinh Quynh, Thanh Tri District, Ha Noi City, Vietnam
3 Plant Protection Research Institute, Duc Thang Ward, Bac Tu Liem District, Ha Noi City, Vietnam
4 Thai Nguyen University of Agriculture and Forestry, Quyet Thang, Thai Nguyen City, Thai Nguyen Province, Vietnam
Hien, P. H., Quynh, B. H., Linh, V. T., Man, L. Q., Nguyen, D. T. & Giang, N. V. (2025). Characterization of siderophore-producing bacteria isolated from plant rhizosphere. Bulg. J. Agric. Sci., 31(2), 372–378
Siderophores are synthesized by plants and microorganisms that thrive in low-iron conditions, serving as biocontrol agents and other applications in agriculture. The aim of the present study was to isolate, characterize, and optimize siderophore-producing bacterial strains DT9, DT10, and DT12. The 16S rDNA nucleotide sequence analysis revealed that DT9 and DT10 were closely related to Klebsiella pneumoniae, and DT12 was related to Acinetobacter bereziniae. All three strains produced siderophores, crucial compounds aiding plants in acquiring iron when growing in iron-scarce environments. The presented results in this study constituted preliminary findings evaluating the influences of cultural conditions on siderophore synthesis by DT9, DT10, and DT12. They demonstrated efficient siderophores synthesis when cultured at 30°C, with a culture medium pH of 7. Glycerol and (NH4)2SO4 served as the carbon and nitrogen sources for the culture medium.