Нordein polymorphism of spring barley genotypes from European-Siberian Genetic Center by SDS-PAGE electrophoresis

Nikolay Neykov1 and Sonya Doneva2
1 Agricultural Academy, Institute of Plant Genetic Resources “Konstantin Malkov”, Sadovo 4122, Bulgaria
2 Agricultural Academy, Dobrudzha Agricultural Institute, Gеneral Toshevo 9500, Bulgaria

Abstract

Neykov, N. & Doneva, S. (2023). Нordein polymorphism of spring barley genotypes from European-Siberian Genetic Center by SDS-PAGE electrophoresis. Bulg. J. Agric. Sci., 29 (1), 97–101

In this study were fractionated and characterized reserve endosperm proteins, hordeins of seven spring barley genotypes with different origin: Zernogradskii (Russia), Bodega, Fink, Scarlett, Barke (Germany), Josefin and Astoria (France) by SDS-PAGE electrophoresis.  On the basis of the obtained spectra are identified 19 bands (D + C + B) with different relative electrophoretic mobility and intensity. The electrophoresis profiles of the groups D-, C- and B-hordein are designated as separate types (models) using the index corresponding to hordein blocks. We have established one profile type for D-hordein (D1), two- for C-hordein (C1, C2), and five – for B hordein (B1, B2, B3, B4, B5). Based on these results are constructed hordein formulas (configurations) of accessions, which enable the expression of specific varietal characteristics and prove the existence of the inter-allelic variation (hordein polymorphism) due to the presence or absence of protein components and their different electrophoretic mobility in the profiles of D-, C- and B-hordein.

Keywords: spring barley; storage proteins; hordein types; intervarietal polymorphism; SDS-PAGE

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