The chemical composition of the n-butanol fraction of the extract of Datura stramonium and its insecticidal activity against pests of the genus Aphididae

S. M. Turaeva1, M. M. Makhmudova1, E. R. Kurbanova1, I. D. Babaev1, R. P. Zakirova1, A. O. Berestetskiy2, A. A. Dalinova2, I. V. Sendersky2 and D. T. Juraev3
1 Academy of Sciences of the Republic of Uzbekistan, Institute of the Chemistry Plant Substances “Acad. S. Yu. Yunusov”, 100170 Tashkent, Uzbekistan
2 All-Russian Institute of Plant Protection, 196608 S. Petersburg, Russian Federation
3 Southern Agricultural Scientific Research Institute, 180100, Karshi, Uzbekistan

Abstract

Turaeva, S. M., Makhmudova, M. M., Kurbanova, E. R., Babaev, I. D., Zakirova, R. P., Berestetskiy, A. O., Dalinova, A. A., Sendersky, I. V. & Juraev, D. T. (2022). The chemical composition of the n-butanol fraction of the extract of Datura stramonium and its insecticidal activity against pests of the genus Aphididae. Bulg. J. Agric. Sci., 28 (5), 901–910

The aim of this work was that the studying the chemical composition of the n-butanol fraction of the extract of the aerial parts of Datura stramonium L. plants collected in the Fergana region of the Republic of Uzbekistan and investigation of its insecticidal activity against certain types of pests of the genus of Aphididae.
It was found as a result of this study that the high efficiency of the n-butanol fraction of the extract of Datura stramonium L. against sucking pests of the genus Aphididae  – Aphis pomi and Macrosiphum euphorbiae. Additionally, 10 mg/mL dose of this fraction also showed the first level activity with 98.5% against Macrosiphum euphorbiae. The level of mortality was lower at 1 mg/mL and 5 mg/mL concentration that 81.3% and 83.3%, respectively. The index was significantly low as a follows -15.1% for 1 mg/mL of the dose, 19.5% for 5 mg/mL of the dose, and 24.0% for 10 mg/mL of the dose when exposed to Schizaphis graminum. It was revealed that the n-butanol fraction of the extract of the aerial part of the Datura stramonium L. plant at a concentration of 1 mg/ml did not show phytotoxicity on the leaves of wheat, tomato, and cucumbers. The first time, it was established that 500 μg / ml and 100 μg/ml concentrations of the fraction showed 100% cytotoxicity against the cell line of maize deciduous scoops (Spodoptera frugiperda) under in vitro conditions and found to be non-toxic to ciliates (Paramecium caudatum) in 5 μl of 10 mg/mL extract.

Keywords: Datura stramonium L.; n-butanol fraction; insecticidal activity; Schizaphis graminum; Aphis pomi; Macrosiphum euphorbiae; phytotoxicity; cytotoxic activity

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