Assessing polymorphism within common bean (Phaseolus vulgaris L.) mutant lines originated from variety “Mastilen 11b” using Inter Simple Sequence Repeats markers

Sibel Aziz1,4, Velichka Spasova-Apostolova2,5, Veselina Masheva3, Nasya Tomlekova1
1 Agricultural Academy, Maritsa Vegetable Crops Research Institute, Breeding Department, 4003 Plovdiv, Bulgaria
2 Agricultural Academy, Tobacco and Tobacco Products Institute, Department of Breeding and Seed Production, 4108 Markovo, Bulgaria
3 Agricultural Academy, Institute of Plant Genetic Resources “Konstantin Malkov”, Department of Plant Genetic Resources, 4122 Sadovo, Bulgaria
4 University of Plovdiv “Paisii Hilendarski”, Faculty of Biology, Department of Developmental Biology, 4000 Plovdiv, Bulgaria
5 Agricultural University, Department of Microbiology and Environmental Biotechnology, 4000 Plovdiv, Bulgaria

Abstract

Aziz, S., Spasova-Apostolova, V., Masheva, V. & Tomlekova, N. (2022). Assessing polymorphism within common bean (Phaseolus vulgaris L.) mutant lines originated from variety “Mastilen 11b” using Inter Simple Sequence Repeats markers. Bulg. J. Agri. Sci., 28 (4), 709–716

The common bean (Phaseolus vulgarisL.) is an economically important crop on a global scale, including in Bulgaria as well. It is an excellent source of vegetable protein and other nutrients. One of the methods of modern plant breeding aimed at creating genetic diversity in beans is induced mutagenesis. Molecular techniques are a suitable tool for characterizing induced mutations. The objective of this work is to evaluate the genetic diversity of a collection of EMS-induced mutant bean lines and to identify specific profiles, using ISSR molecular markers. The initial variety “Mastilen 11b” and 15 mutant lines from the collection of the Maritsa Vegetable Crops Research Institute are analyzed with 14 ISSR reactions. A total of 187 fragments with lengths from 190 bp to 3100 bp are generated. Three of the reactions (ISSR 6, ISSR 8, ISSR 9) led to a generation of monomorphic profiles, and the remaining eleven reactions (ISSR 1, ISSR 2, ISSR 2T, ISSR 3, ISSR 4, ISSR 5, ISSR 7, ISSR 10, ISSR 12, UBC 807, UBC 809) amplified polymorphic profiles. The polymorphism ranged from 18.65 % to 87.50 % in the analyzed genotypes. The results showed that the applied ISSR markers are effective for assessing the existing genetic diversity of the studied bean mutant lines.

Keywords: Phaseolus vulgaris L., mutant lines, ISSR markers

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