Characteristics of a hybrid (F1 A. baerii x A. gueldenstaedtii) sperm production at different ages when grown on an industrial cage farm

Stanimir Bonev1, Boyko Georgiev2, Lyudmila Nikolova1, Paulina Taushanova2
1 Agricultural University, Faculty of Agronomy, Department “Animal science”, BG-4000 Plovdiv, Bulgaria
2 Institute of Biology and Immunology of reproduction “Acad. K. Bratanov”, BAS, Sofia, Bulgaria

Abstract

Bonev, S, Georgiev, B, Nikolova, L., Taushanova, P. (2022) Characteristics of a hybrid (F1 A. baerii x A. gueldenstaedtii) sperm production at different ages when grown on an industrial cage farm. Bulg. J. Agric. Sci., 28 (Supplement 1), 164–171

The study was performed with male hybrid individuals (F1 Acipenser baerii x Acipenser gueldenstaedtii) aged seven, eight and nine years, grown on a cage farm located in a warm water dam. No significant differences were found between age groups in ejaculate volume and sperm concentration. Motility analysis showed that sperm in the oldest individuals had the highest values (p < 0.05) of VCL, VSL, VAP, ALH and BCF, while the differences in indicators were not significant between the hybrids at 7- and 8 years of age. The relative proportion of rapid sperm in the hybrid semen of varies from 0 to 96.90%; of medium sperm from 1.0 to 46.74%; slow from 0.28 to 75.30%; static from 0 to 84%. The relative share of spermatozoa with non progressive motility varies from 15.30 to 89.70%, and those with progressive motility – from 0.10 to 29.85%. The oldest fish had significantly (p < 0.05) the best characteristics of sperm motility – the highest share of rapid and those with progressive motility; lowest of the slow and static. Age affects the enzymatic activity of sperm. In 7- and 9-year-old fish, a significant difference was found in AP, GGT, CK in water extract, and in LDH in triton extract. The water extract in 9-year-olds has higher levels of AP (7 times; p < 0.05) and GGT (3.2 times; p <0.05), while in 7-year-olds fish the values of CK are higher (2.3 times; p < 0.05). In LDH, the difference (1.5 times) is also in favor of 7-year-old individuals, but it is not significant. In the triton extract only AP levels are higher (over 30%) in 9-year-old fish, but the difference is not significant. For the other indicators (GGT, CK and LDH) the difference is in favor of the younger fish, respectively 4.6 %, 22.1 % and 2.9 times, and for the latter it is statistically significant (p < 0.05).

Keywords: sturgeon semen, enzyme activity, spermatozoa velocity.
Abbreviations: CASA – Computer-assisted sperm analysis; VCL – curvilinear velocity; VSL – straight line velocity; VAP – average path velocity; LIN – linearity; STR – straightness; WOB – wobble; ALH – amplitude of lateral head displacement; BCF – beat/cross frequency; AP – Alkaline Phosphatase; GGT – Gamma-glutamyl Transferase; CK – Creatine Kinase; LDH – Lactate Dehydrogenase.

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