European grayling phylogeographic lineages of Russian European North from barcoding DNA fragment

Ekaterina V. Ponomareva1, Aleksandr A. Volkov2, Maria V. Ponomareva1, Galina A. Makeenko3 and Elena A. Shubina4
1 Lomonosov Moscow State University, Faculty of Biology, Moscow, 119234, Russia
2 Russian Federal Research Institute of Fisheries and Oceanography, Moscow, 107140, Russia
3 Murmansk Arctic State University, Murmansk, 183038, Russia
4 Lomonosov Moscow State University, Belozersky Research Institute of Physico-Chemical Biology, Moscow, 119991, Russia

Abstract

Ponomareva, E. V., Volkov, A. A., Ponomareva, M. V., Makeenko, G. A. & Shubina, E. A. European grayling phylogeographic lineages of Russian European North from barcoding DNA fragment. Bulg. J. Agri. Sci., 28, (Supplement), 111–118

MtDNA CO1 barcoding fragment was analyzed in 302 European graylings (Thymallus thymallus L.) from 38 localities of the Russian European North. Four T. arcticus Pallas haplotypes were found in 62 fishes from the rivers of the Kola Peninsula and 3 fishes from the Northern Dvina basin; that indicates an ancient introgressive hybridization of European and Arctic graylings. Two T. thymallus haplotypes differing by two substitutions dominated in most of studied fishes and formed two haplogroups, one of which dominates in most water bodies of the Kola Peninsula, Pechora and some tributaries of the Northern Dvina. In the northeast, another haplogroup dominated and might be diagnostic for the identification of Russian European North European grayling. The observed distribution of haplotypes reflects post-glacial colonization and is associated with paleoclimatic changes that fragmented the grayling range.
Thus, CO1 barcoding fragment can be used to identify origin of grayling and is suitable for describing phylogeographic lineages and indicating evolutionary significant units (ESU).

Keywords: European grayling; T. thymallus L.; arctic grayling; T. arcticus Pallas; introgressive hybridization; CO1; Russian European North

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