Effect of three extenders on the motility and morphological characteristics of spermatozoa in diluted Muscovy semen stored at 4°C up to 120 hours

Vasko Gerzilov1 and Madlena Andreeva2,3
1 Agricultural University, Department of Animal Science, 4000 Plovdiv, Bulgaria
2 Bulgarian Academy of Sciences, Institute of Biology and Immunology of Reproduction “Acad. Kiril Bratanov”, 1113 Sofia, Bulgaria
3 Bulgarian Academy of Sciences, Institute of Neurobiology, 1113, Sofia, Bulgaria

Abstract

Gerzilov, V. & Andreeva, M. (2021). Effect of three extenders on the motility and morphological characteristics of spermatozoa in diluted Muscovy semen stored at 4°C up to 120 hours. Bulg. J. Agric. Sci., 27 (6), 1187–1193

The present study evaluated the total and progressive motility, as well as sperm morphological characteristics of diluted semen with three different extenders (1:3 v/v) and stored at 4°C for 3, 6, 24, 30, 48, 54, 72, 78, 96, 120 h. Total (TSM) and progressive mobility (PSM) gradually decrease with prolonging storage time. Up to the 30 h of sperm storage the sperm maintain high TSM – over 80% in semen diluted with IMV Canadyl and AU extenders and over 68% when diluted with HIA-1 extenders. With increasing in vitro sperm storage time, PSM and rapid sperm cells decrease significantly after the 3rd h, while non-progressive motility increases until the 48th h, after which it also begins to decrease. For velocity parameters VCL, VAP, VSL are reported a more rapid trend of decrease between 3 and 30 h of cold storage, after which the decline is smoother (P < 0.05). As storage time is extended, the pathological forms of the sperm cells increase, with those affecting the head of the sperm cells predominating – crooked-neck and swelling forms.

Keywords: Muscovy duck; semen; extender; liquid storage; sperm motility; sperm morphology

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