Dora Trifonova1, Ivanka Stoilova1, Andrey Marchev2, Petko Denev3, Galena Angelova1, Anna Lante4 and Albert Krastanov1
1 University of Food Technology, Department of Biotechnology, 4002 Plovdiv, Bulgaria
2 Bulgarian Academy of Sciences, Department of Applied Microbiology, Laboratory of Applied Biotechnologies, 4000 Plovdiv, Bulgaria
3 Bulgarian Academy of Sciences, Institute of Organic Chemistry with Centre of Phytochemistry, Laboratory of Biologically Active Substances, 4000 Plovdiv, Bulgaria
4 Università di Padova, Department of Agronomy, Food, Natural Resources, Animals and Environment, Viale dell`Universita 16, Agripolis, 35020 Legnaro, Padova, Italy
Trifonova, D., Stoilova, I., Marchev, A., Denev, P., Angelova, G., Lante, A. & Krastanov, A. (2021). Phytochemical constituents of pressurized liquid extract from Ziziphus jujubа Mill. (Rhamnaceae) fruits and in vitro inhibitory activity on α-glucosidase, pancreatic α-amylase and lipase. Bulg. J. Agric. Sci., 27 (2), 391–402
In the current study the phytochemical composition of extract from Ziziphus jujubа Mill (Rhamnaceae) fruits obtained by pressurized liquid extraction (PLE) was investigated. The phytochemical analysis showed the presence of triterpenes, flavonoids and phenolic acids. The fruit extract had a total phenolic content of 21 ±2.01 mg gallic acid equivalents per g of extract (mg GAE/g extract), and total flavonoid content 2.48 ± 0.24 mg catechin equivalents per g dried extract (mg CE/g extract). This study evaluated the in vitro antioxidant activity of jujube extract and its inhibitory effects on α-glucosidase and pancreatic α-amylase and lipase. The obtained results proved inhibition of α-glucosidase with 70.94% and inhibition of α-amylase with 56.08%. Enzyme kinetic studies indicated that the inhibitors in extract have the most potent inhibition of lipase compared to other two enzymes with inhibitory constant Ki of 0.074 mg/cm3. These results suggest the possible application of extract from Z. jujubа fruits obtained by PLE in the management of metabolic disorders as a whole. These are the first studies on this plant species cultivated in Bulgaria.