Antimicrobial activity of methanolic and ethanolic crude extracts of carp, peel and seed of Punica granatum L.

Emad I. Hussein1,3, Wesam Al Khateeb1, Mutaz A. Abd Al-Razaq 1, Jacob H. Jacob2, Khalaf Alhussaen4 and Abdul-Salam F. Juhmani1
1 Yarmouk University, Department of Biological Sciences, 21163, Irbid, Jordan
2 Al al-Bayt University, Department of Biological Sciences, 130040, Al-Mafraq, Jordan
3 A’Sharqiyah University, College of Applied and Health Sciences, Department of Food Science and Human Nutrition, Ibra, 42, Oman
4 University of Tabuk, College of Hagel, Department of Biology, 71491, Tabuk, Saudia Arabi


Hussein, E. I., Al Khateeb, W., Abd Al-Razaq, M. A., Jacob, J. H., Alhussaen, K. & Juhmani, A.-S. F. (2021) Antimicrobial Activity of Methanolic and Ethanolic Crude Extracts of Carp, Peel and Seed of Punica granatum L. Bulg. J. Agric. Sci., 27(1), 227–233

Punica granatum L. (pomegranate) is an ancient fruit that is widely consumed as fresh fruit and juice. In vitro and in vivo studies have demonstrated that this fruit possesses antioxidant, antidiabetic, hypolipidemic, antibacterial, anti-inflammatory, and antiviral activities. In this study, the antimicrobial activities of two types of cultivars (sour and sweet) were tested. Therefore, methanolic and ethanolic extracts of different fruit parts (carp, peel, and seed) of Punica granatum L. were prepared and tested against four Gram-negative bacteria species, three Gram-positive bacteria species, and three fungal species using agar diffusion method as well as broth dilution method. The maximum value of the inhibition zone (40 mm) was obtained by ethanolic peel crude extract of sweet P. granatum against the Gram-positive Micrococcus luteus. The minimum inhibition zone (10 mm) was obtained, however, by an ethanolic crude extract of P. granatum against Salmonella typhimurium. The minimal inhibitory concentrations (MIC) of P. granatum crude extracts showed that methanolic peel crude of sour P. granatum had the lowest MIC value (10 µg/ml) against Serratia marcescens. In contrast, the highest MIC value (360 µg/ml) was in case of methanolic seed crude of P. granatum against Micrococcus luteus.

Keywords:Punica granatum; carp, peel; seed; antimicrobial; methanolic, ethanolic

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