Ralitsa Balkanska1, Katerina Stefanova2, Radostina Stoikova-Grigorova3 and Maya Ignatova1
1Agricultural Academy, Institute of Animal Science, Department of Special Branches, 2230 Kostinbrod, Bulgaria
2Agricultural Academy, Agrobioinstitute, Molecular Genetics, 1164 Sofia, Bulgaria
3Agricultural Academy, Institute of Animal Science, Department of Genetics, 2230 Kostinbrod, Bulgaria
Balkanska, R., Stefanova, K., Stoikova-Grigorova, R. & Ignatova, M. (2020). A preliminary assessment of trnH-psbA as DNA barcode for botanical identification of polyfloral honey samples and comparison with rbcL marker. Bulg. J. Agric. Sci., 26 (1), 238–242
Honey is a natural and sweet product produced from Apis mellifera L. It can be classified as monofloral and polyfloral. DNA barcoding of pollen in honey can be used to identify the source of honey with high efficiency. Three polyfloral honey samples were used in the study. The trnH-psbA barcode was found to be an effective marker as source of additional date in identifying of the botanical origin of honey samples with high level of confidence. The purpose of this study was first to assess the efficiency of trnH-psbA marker as a DNA barcode for botanical identification and to compare the data with the obtained results for rbcL marker as a complex approach for botanical identification of polyfloral honey samples.