Indigenous phosphate-solubilizing bacteria enhance germination in deteriorated rice seed

Purwaningsih1, Radian2, Widyatmani Sih Dewi3, Bambang Pujiasmanto3
1 Sebelas Maret University, Doctoral Program of Agriculture Science, Graduate School, Jalan Ir. Sutami 36, Surakarta Surakarta, Central Java, Indonesia
2 Tanjungpura University, Agricultural Faculty, Kalimantan Barat 78115, Indonesia
3 Sebelas Maret University, Agricultural Faculty, Jalan Ir. Sutami 36, Surakarta, Central Java, Indonesia


Purwaningsih, Radian, Dewi, W. & Pujiasmanto, B. (2019). Indigenous phosphate-solubilizing bacteria enhance germination in deteriorated rice seed. Bulgarian Journal of Agricultural Science, 25(3), 486–493

Certain soil microorganisms have the potential to improve the growth of plants. The aim of this study was to isolate the indigenous bacteria from acid sulphate soil, and evaluate whether this could enhance the germination of deteriorating rice seeds. It was revealed that four bacteria isolates (4A, 6A, 8RH, 10RH) were able to solubilise phosphate and produce indole and siderophore. Molecular identification, using the 16S rRNA gene, showed that these four isolates are Paenibacillus alvei strain NBRC 3343, P. alvei strain DSM 29, Bacillus cereus strain ATCC 14579 and B. cereus strain ATCC 14579. Evaluation showed that the four strains were able to increase the percentage of germination by up to 46.5%, the uniformity of germination by up to 30% and the germination rate by 7.1%/d on seed with ≤ 50% viability. Germination of seeds with 80% viability increased by only 14%, with a slight increase in the uniformity of germination (16%) and germination rate (8.81%/d).

Keywords: indigenous; phosphate-solubilising bacteria; deteriorated rice seed; germination; 16S rRNA gene; indole; siderophore

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