Efficiency of lactic acid bacteria as the potent degradative microorganism to digest the total mixed ration in vitro

Wichai Suphalucksana1, Kasem Soytong2
¹ Department of Animal Production Technology and Fishery, Faculty of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, 10520 Thailand
² Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, 10520 Thailand

Abstract

Suphalucksana, W., & Soytong, K. (2018). Efficiency of lactic acid bacteria as the potent degradative microorganism to digest the total mixed ration in vitro. Bulgarian Journal of Agricultural Science, 24(6), 1104–1108

The objective of this research was to investigate the efficiency of lactic acid bacteria on in vitro digestibility of total mixed ration (TMR) containing pineapple plant silage 55%, rice straw 15%, cassava ship 5.6%, soybean meal 12.6%, palm kernel pulp l4.2%, rice bran 5.6%, premixed 0.15% and molasses 2%. Guinea grass (Panicum maximum) was used for isolation and identification of microorganisms from silage. Treatments were used as TMR treated with lactic acid bacteria as follows: Treatment 1 – non treated; Treatment 2 – TMR treated with Lactobacillus pantarum;Treatment 3 – TMR treated with Pediococcus pentosaceus;Treatment 4– TMR treated with Pediococcus acidilactici; Treatment5 – TMR treated with L. pantarum  + P. pentosaceus  + P. acidilactici. The treated TMR were mixed and put in polyethylene bags and stored at ambient temperature for 7 days before analysis. The in vitro digestibility test used pepsin cellulase in vitro digestibility method. The results showed that there were significant differences (P < 0.01) of the nutritive value of TMR by proximate analysis and fiber analysis which treated lactic acid bacteria. For the digestibility, results showed that the TMR treated with LAB using pepsin cellulase in vitro digestibility which applied LP, PP, PA and LPP could improve the digestibility of TMR, which significantly increased in digestibility of TMR (P < 0.01). The rate of increase and decrease of digestibility and fiber in TMR treated with LAB showed an increase in dry matter and a decrease in fiber. Therefore, it was suggested that TMR treated with lactic acid bacteria of TMR may possible to develop as biological animal feed for ruminant.

Keywords: lactic acid bacteria; digestibility; total mixed ration
Abbreviations: TMR – total mixed ration; LAB – lactic acid bacteria; CRD – completely randomized design; DM – dry matter; ASH – ash; CF – crude fat; EE – ether extract; CP – crude protein; CF – crude fiber; AIA – acid insoluble ash; NDF – neutral detergent fiber; ADF – acid detergent fiber; ADL – acid detergent lignin; DMRT – Duncan’s Multiple Range Test; LP – Lactobacillus pantarum; PP – Pediococcus pentosaceus; PA – Pediococcus acidilactici; LPP – L. pantarum  + P. pentosaceus  + P. acidilactici

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