Svetla Yancheva1, Petar Marchev1,2, Veneta Yaneva2, Venelin Roichev1, Ivan Tsvetkov3
1 Agricultural University, 12 Mendeleev Str., 4000 Plovdiv, Bulgaria
2 AMV AGRO, 4023 Plovdiv, Bulgaria
3 AgroBioInstitute, 8 Dragan Tsankov Blvd., 1164 Sofia, Bulgaria
Yancheva, S., Marchev, P., Yaneva, V., Roichev, V. & Tsvetkov, I. (2018). In vitro propagation of grape cultivars and rootstocks for production of pre-basic planting material. Bulgarian Journal of Agricultural Science, 24(5), 801–806
An optimized and simplified procedure for in vitro propagation of pre-basic planting material from different grape cultivars and rootstocks has been developed. A significant advantage of the applied propagation system was that the multiplication and rooting phases occurred simultaneously. The use of the specially modified nutrient medium provided an economically viable production of healthy and hardened plants. It was shown that the genotype is the primary factor determining a high multiplication and economically important efficiency in this process. Applying the optimized micropropagation system, the studied grape genotypes have been successfully propagated in vitro and acclimatized to ex vitro conditions. Obtained pre-basic plant material of more than 2500 plantlets was cultivated in a nursery for future investigation.