KANATBEK MUKANTAYEV1; KANAT TURSUNOV1; BAKHYTKALI INGIRBAY1; ZHANSAYA ADISH1; MALIKA AZHIBAYEVA1; ZHULDYZ KAIROVA1; ERLAN RAMANKULOV2; KASYM MUKANOV1; ALEXANDER SHUSTOV3
1 National Center of Biotechnology, Laboratory of Immunochemistry and Immunobiotechnology, 010000, Astana, Kazakhstan
2 National Center of Biotechnology, 010000, Astana, Kazakhstan
3 National Center of Biotechnology, Laboratory of Genetic Engineering, 010000, Astana, Kazakhstan
Mukantayev, K., K. Tursunov, B. Ingirbay, Z. Adish, M. Azhibayeva, Z. Kairova, E. Ramankulov, K. Mukanov and A. Shustov, 2018. Immunochromatographic assay for the foot-and-mouth disease utilizing recombinant nonstructural proteins 2C, 3A, 3B and 3D. Bulg. J. Agric. Sci., 24 (3): 489–496
Foot-and-mouth disease (FMD) is a highly contagious infection of domestic and wild cloven-hoofed animals. Recommended measures to prevent transmission of the FMD from endemic areas is a vaccination of livestock in endangered territories and creation of a buffer zone in which susceptible animals are vaccinated. Because FMD vaccines do not provide long protective immunity control for possible infection in vaccinated animals is important in areas with vaccination. It is possible by using DIVA (Discrimination of Infected from Vaccinated Animals) assays which detect antibodies to non-structural proteins (NSPs) of the FMD virus. The paper describes development of an immunochromatographic assay (ICA) which uses recombinantly expressed NSPs 2C, 3A, 3B and 3D as antigens to capture specific antibodies. The recombinant FMD antigens were produced using expression in E. coli and purified by metal affinity column chromatography. Sensitivity and specificity of the ICA were found to be 92-97% and 91-96%, respectively in samples of sera from animals with varying risk of the FMD.