SUPHALUCKSANA WICHAI1; KASEM SOYTONG2
1 King Mongkut’s Institute of Technology Ladkrabang, Department of Animal Production Technology and Fishery, Faculty of Agricultural Technology, Bangkok, 10520 Thailand
2 King Mongkut’s Institute of Technology Ladkrabang, Department of Plant Production Technology, Faculty of Agricultural Technology, Bangkok, 10520 Thailand
Wichai, S. and K. Soytong, 2017. Lactic acid bacteria and enzyme production in silage of guinea grass (Panicum maximum). Bulg. J. Agric. Sci., 23 (1): 86–91
Two different isolates of lactic acid bacteria, Lactobacillus plantarum H5-M13F and Pediococcus pentosaceus Ac2-M13F were confi rmed by morphological and phylogenic identifi cation. H5-M13F was rod shaped, gram positive, negative for gas production and catalase activity, homofermentative, and grew at 10 – 45 C, pH 3.5 – 9.6, and 1.0 – 10 % NaCl2. Isolate Ac2-M13F was coccus shaped, gram positive, negative for gas production and catalase activity, homofermentative, and grew at 10 – 45 C, pH 3.5 – 9.6, and 1.0 – 6.5 % NaCl2. Amplifi cation of the ITS regions from isolates H5-M13F and Ac2-M13F were confi rmed by using Primer OPA-3. The phylogenetic tree from PAUP analysis indicated that isolate H5-M13F clustered with Lactobacillus sp. and isolate Ac2-M13F with Pediococcus sp. Phylogenetic analysis and dendrograms revealed no relationship between the two groups. It was found that isolate H5-M13F mostly isolated from silages (87.59%) while isolate Ac2-M13F was found at a low incidence in silages (3.19%). Lactobacillus plantarum H5-M13F and Pediococcus pentosaceus Ac2-M13F from silage of guinea grass showed the ability to produce extracellular degradative enzymes. Pediococcus pentosaceus Ac2-M13F produced amylase and protease and Lactobacillus plantarum H5-M13F produced protease.