M. BOZHILOVA-SAKOVA1, I. DIMITROVA1, A. TENEVA1 and N. PETROV2
1 University of Forestry, Department of Genetics and Selection, Agronomy Faculty, 10, Kl. Ohridski bl.,1756 Sofia, Bulgaria
2 Institute of Soil Science “Nikola Poushkarov” 7, Shousse Bankya Str., 1080 Sofia, Bulgaria
Bozhilova-Sakova, M., I. Dimitrova, A. Teneva and N. Petrov, 2016. PCR-RFLP analysis of MSTN gene in Karakachan sheep breed. Bulg. J. Agric. Sci. 22 (Suppl. 1): 115–117
The present study was conducted in order to investigate and identify the allelic variants of myostatin (MSTN) gene associated with meat traits in sheep. Blood samples were taken from a population of 25 animals of Bulgarian breed Karakachan sheep – 22 ewes and 3 rams. Genomic DNA was extracted and myostatin genotypes were estimated by means of PCR amplification and PCR-RFLP method. The PCR products were digested with HaeIII restriction enzyme. In the total population of sheep polymorphism was not found. The MSTN locus was found to be monomorphic – only the allele m and the genotype mm were detected.