Lycopene Quantification of Tomato by SPE and HPLC

Shoolini University of Biotechnology and Management Sciences, Department of Microbiology, Bhajol-Solan, (HP)-173213, India


THAKUR, N., 2016. Lycopene quantification of tomato by SPE and HPLC. Bulg. J. Agric. Sci., 22: 84–90

Indiscriminate use of chemical fertilizers and pesticides could cause adverse changes in biological balance as well as lead to an increase in incidence of cancer and other diseases, through the toxic residues present in the grains and other edible parts. Keeping in view the above criteria, the present studies were carried out in the farmers field (village Basal), 5 km away from Solan town, under mid hill conditions of Himachal Pradesh during the two consecutive years (2013 and 2014), to study the effect of different organic and inorganic treatments on lycopene content of tomato (cv. Solan Lalima). A Field trial was laid out in a randomized block design (RBD) with seven treatments replicated thrice. The results revealed that the combination of organic manures and biofertilizers proved best to improve the lycopene content over the farmer’s system of cultivation (conventional treatment). The lycopene extracted by SPE (Solid Phase Extraction) column and further purified by HPLC analysis of organic sample showed maximum increase of 19.66% and 11.06% in lycopene content over control. It can be concluded from the present studies that by adopting appropriate combination of organic production technologies, tomato with better nutritional quality can be easily cultivated

Key words: tomato; organic; conventional; lycopene; SPE; HPLC
Abbrevations: FYM: Farm Yard Manure; VC: Vermicompost; AZO: Azotobacter; PSB: Phosphate Soluabalizing Bacteria; SPE: Solid Phase Extraction; HPLC: High Performance Liquid Chromatography; RT: Retention Time

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