High-Frequency in vitro Direct Shoots Regeneration from Axillary Nodal and Shoot Tip Explants of Clary Sage (Salvia sclarea L.)

1 Urmia University, Faculty of Agriculture, Department of Horticultural Science, Nazlo Capmus, P.O.Box: 165, Urmia, Iran
2 National Institute of Genetic Engineering and Biotechnology


GHANBAR, T., B. HOSSEINI, Z. JABBARZADEH, A. FAROKHZAD and A. SHARAFI, 2016. High-frequency in vitro direct shoots regeneration from axillary nodal and shoot tip explants of clary sage (Salvia sclarea L.). Bulg. J. Agric. Sci., 22: 73–78

In vitro bud induction and shoot regeneration of Salvia sclarea L. was investigated. In vitro regeneration was achieved on MS media supplemented with various combinations of BAP, TDZ and IAA. In the first phase of the experiment, the interaction between explant type (shoot tip and axillary node) and plant growth regulators (TDZ and BAP) (0, 2.2, 4.4 and 8.8 μM) on bud induction and plant regeneration was investigated. According to the results, the interaction of explants and growth regulators on bud induction and regeneration of shoots on the 5% level was not significant. Maximum average of bud induction and shoot regeneration was achieved in axillary nodal explants. Compare data obtained from the effect of TDZ and BAP on the induction of buds and regenerated shoots showed that in culture media supplemented with different concentrations of the TDZ in compared with the same concentration of BAP, bud induction and shoot regeneration was better. In the second experiment, the effect of axillary bud explants in culture media containing different concentrations of TDZ (0, 2.2, 4.4 and 8.8 μM) in combined with 1.1 μM IAA were studied. Results showed that the maximum average of bud induction and shoot regeneration were in media containing 2.2 μM TDZ in combined with IAA. Root induction also was evaluated on ½ MS and MS media with two concentrations (0.5 and 1 mgl-1) of IAA or IBA. MS media supplemented with IAA (0.5 mgl-1) was the best medium for rooting.

Key words: bud induction, rooting, Salvia sclarea, shoot proliferation
Abbreviations: MS - Murashige and Skoog, BAP - 6-Benzylaminopurine, TDZ - Thidiazuron, IAA - Indole-3 Acetic Acid; IBA - Indole-3 Butyric Acid

See the article as a PDF.