Development of Root Tips Synchronized System for the Model Legume Medicago truncatula upon Replication Stress

AgroBioInstitute, BG - 1164 Sofia, Bulgaria


IANTCHEVA, A., I. BOYCHEVA and M. REVALSKA, 2015. Development of root tips synchronized system for the model legume Medicago truncatula upon replication stress. Bulg. J. Agric. Sci., 21: 1177–1184

Up to now the synchronized cell cultures were developed for alfalfa (Medicago sativa) and they served as a valuable tool for the identification and understanding of key regulators of the cell cycle. These cell culture systems were characterized with some disadvantages like continuous use of mitogenic compounds and conditions that differ from the stable physiological state in the plant meristem cells. The present procedure, described in this study is an easy and fast system for root tips synchronization of Medicago truncatula, upon hydroxyurea treatment. The system was originally developed for Arabidopsis thaliana and adapted for the model legume. The present study opens up possibility to investigate gene of interest that are involved in Medicago truncatula cell cycle progression.

Key words: Medicago truncatula, cell cycle, root tips synchronization system, cyc like F-box protein
Abbreviations: 2,4-Dichlorophenoxyacetic acid; BAP 6-Benzylaminopurine; NAA a-Naphthaleneacetic

See the article as a PDF.