Pyramiding of Four Transgenes in One Tobacco Line by Sexual Crosses

AgroBioInstitute, BG - 1164 Sofia, Bulgaria


CHRISTOVA, P. and R. BATCHVAROVA, 2015. Pyramiding of four transgenes in one tobacco line by sexual crosses. Bulg. J. Agric. Sci., 21: 829–834

To combine four transgenes (Np, CP, ttr and ahas 3R) in one tobacco line a cascade of sexual crosses between four transgenic tobacco lines resistant to TSWV, PVY, P. syringae pv. tabaci and herbicide Glean®, respectively, was performed. The first step of the experiment where genes for resistance were combined in pairs was previously described (Docheva et al., 2002). Here we report the second step of pyramiding of four transgenes and combining the resistance to TSWV, PVY, P. syringaepv. tabaci and herbicide Glean® in one tobacco line (cross 2002). Complex resistance to all tree pathogens and the herbicide was analyzed in F1 and F2 generations and integration of all four transgenes in tobacco genome was proved by PCR analyses. Thirteen F2 tobacco lines with pyramided genes for resistance to TSWV, PVY, P. syringae pv. tabaci and herbicide Glean® were selected.

Key words: TSWV, PVY, P. syringae pv. tabaci, herbicide, cross pollination, resistance

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