Influence of Nutritive Media and Low Temperatures on Broomrape Seed Germination

ST. MASIREVIC¹, S. MEDIC-PAP², B. KONSTANTINOVIC¹ and A. TERZIC^1
1 Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia
² Institute of Field and Vegetable Crops, Novi Sad, Serbia

Abstract

MASIREVIC, ST., S. MEDIC-PAP, B. KONSTANTINOVIC and A. TERZIC, 2015. Influence of nutritive media and low temperatures on broomrape seed germination. Bulg. J. Agric. Sci., 21: 100-104

The germination of broomrape seeds on different nutritive media and at low temperatures was tested. Broomrape seed was collected from three localities in Northern part of Serbia. Nutritive media which were used are: water agar, water agar with gibberelic acid and water agar with biological agent Trifender. Temperature regimes were: without cooling, and in the fridge for 7, 14 and 21 days at + 4°C. The highest number of germinated seeds was observed on the agar medium with gibberelic acid in presence of sunflower roots. Cooling of broomrape seeds on 4°C during 21 day leads to stimulation of their germination and average radicle length. The effect of bio-agent Trifender should be further investigated.

Key words: broomrape, seed germination, sunflower, nutritive media
Abbreviations: water agar (WA); water agar with giberellic acid (GA); water agar medium with Trifender (T); WA-B, GA-B nutritive media (WA or GA) without cooling seeds (4°C); WA-7, GA-7 nutritive media (WA or GA) with cooling seeds (4°C) for 7 days; WA-14, GA-14 nutritive media (WA or GA) with cooling seeds (4°C) for 14 days; WA-21, GA-21– nutritive media (WA or GA) with cooling seeds (4°C) for 21 days

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