Staphylococcus aureus and Other Pathogens in Relation to Breed of Cattle and Somatic Cell Count

1 Dairy Research Institute, Ke Dvoru 12a, Prague, Czech Republic
2 Agriresearch Rapotín, Výzkumníků 267, Vikýřovice, Czech Republic
3 Agritec Plant Research, Zemědělská 16, Šumperk, Czech Republic


VYLETELOVA-KLIMESOVA, M., O. HANUS, A. DUFEK, I. NEMECKOVA, J. HORACEK, A. PONIZIL and L. NEJESCHLEBOVA , 2014. Staphylococcus aureus and other pathogens in relation to breed of cattle and somatic cell count. Bulg. J. Agric. Sci., 20: 1477-1482


Influence of breed on somatic cell count (SCC) and occurrence of particular species was evaluated. The samples were collected from farms with Holstein (H; 10 farms - 365 cows) breeding and from farms with Czech Fleckvieh (CF; 2 farms – 67 cows). The obtained dataset was statisticaly evaluated by analysis of variance. The occurrence of pathogens was compared between H and CF. The relationship between SCC and incidence of pathogens was determined as well. It is evident that more positive cows were in breed H compared to CF (H 41.6%; CF 26.9%). The most frequent pathogens in H breed were S. aureus(13.7%), S. uberis (9.6%), S. haemolyticus (8.5%), S. agalactiae (6.9%), E. faecalis (2.5%) and E. faecium (0.8%), while only S. uberis (19.4%), S. haemolyticus (6%) and S. aureus (1.5%) were found in CF. SCC results showed the higher SCC in H breed in case of both negative and positive cows as well and significant impact of breed on logSCC (F = 6.4, P = 0.012). The significant effects of bacterial species were confirmed (F = 13.6, P < 0.001). Multiple comparison among groups of bacterial species showed significant differences between negative and S. aureus, S. agalactiae, S. uberis (P = 0.012, P < 0.001, P < 0.001). Differences between negative and other pathogens were not signifiant.

Key words: raw milk; Holstein; Czech Fleckvieh; pathogens; somatic cell count
Abbreviations: H - Holstein, CF - Czech Fleckvieh, SCC - somatic cell count, TCM - total count of microorganisms, BC - Brown cattle, SC - Simmental cattle, J - Jersey, CSN - Czech Norm Standards, MRSA - methicillin-resistant Staphylococcus aureus, SPSS - Statistical Package for the Social Sciences, ANOVA - Analysis of variance , IMI - intramammary infection, SCS - somatic cell score, n - count, Tukey´s HSD - Tukey‘s honestly significant difference test, SAU - Staphylococcus aureus, SA G - Streptococcus agalactiae, SHA - Staphylococcus haemolyticus, SUB - S. uberis, ENT - E. faecalis and E. faecium, NEG - negative, xg - geometric mean, P - level of significance (probability of zero hypothesis validity), F - value of F-test criterion, CI - confidence interval, d – difference

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