New Sunflower (H. annuus L.) Lines as Results of Interspecific and Intergeneric Hybridization And Application of Method of Direct Organogenesis in F1 Immature Embryo

1 Dobroudja Agricultural Institute, BG – 9520 General Toshevo, Bulgaria
2 Institute of Plant Growing and Plant Protection, Giesen, Germany


ENCHEVA, J., H. KÖHLER, M. CHRISTOV, P. SHINDROVA, V. ENCHEVA and W. FRIEDT, 2014. New sunflower (H. annuus L.) lines as results of interspecific and intergeneric hybridization and application of method of direct organogenesis in F1 immature embryo. Bulg. J. Agric. Sci., 20: 1444-1449


Тhe direct organogenesis method in immature F1 embryos from sunflower was successfully applied for production of new forms from the interspecific crosses H. annuus (cv. Albena) x H. tuberosus, H. annuus (cv. Albena) x H. salicifolius and intergeneric cross Helianthus annuus (cv. Albena) x Verbisina helianthoides (genera Verbisina). After repeated selfing and continuous selection, a great diversity of new sunflower lines were developed. The available literature does not provide data on the obtaining of these hybrid combination by direct organogenesis method. The applied molecular method (RAPD) confirmed the hybrid nature of the obtained breeding material and indicated an introgression of H. tuberosus and V. helianthoides DNA into some of the hybrid progenies produced. Our results are also the first attempt to investigate the combining ability of the new restorer lines produced through interspecific and intergeneric hybridization. Some of the new lines possess resistance to important diseases and parasite broomrape and very good combining ability. The new hybrids developed showed an increase of the indexes seed yield and oil yield in comparison to the mean standard (commercial hybrids Albena and Super start).

Key words: Helianthus annuus, H. tuberosus, H. salicifolius, Verbisina helianthoides, organogenesis, new breeding material, RAPD analysis, resistance, Phomopsis helianthi, Phoma macdonaldii, Alternaria helianthi, Plasmopara helianthi, Orobanche cumana

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