Evaluation and Comparison of ISSR and RAPD Markers for Assessment of Genetic Diversity in Triticale Genotypes

Department of Field Crops, Faculty of Agriculture, Ege University, Bornova, Izmir, Turkey


TONK, F. A., M. TOSUN, E. ILKER, D. ISTIPLILER and O. TATAR, 2014. Evaluation and comparison of ISSR and RAPD markers for assessment of genetic diversity in triticale genotypes. Bulg. J. Agric. Sci., 20: 1413-1420


Assessments of genetic diversity in a gene pool facilitate effective selection and shorten the breeding time. Two PCRbased marker approaches, Inter-simple sequence repeat (ISSR) and Random amplified polymorphic DNA (RAPD) were used to evaluate genetic diversity of 16 triticale genotypes grown in Turkey and relative efficiencies of the marker systems were compared. From 28 and 21 primers were utilized in ISSR and RAPD markers, respectively. Effective multiplex ratio (EMR), marker index (MI), resolving power (Rp) and polymorphic information content (PIC) of the primers were calculated for the two marker systems and all the parameters examined found to be higher in ISSR system. The Mantel test revealed a highly significant correlation (r = 0.74) between the Jaccard’s similarity matrices of RAPD and ISSR. The most remarkable result of the current study is that cluster analysis on ISSR and RAPD data clearly discriminated the genotypes in terms of their growth habit and origin, respectively and triticale genotypes were clustered from two different points of view and both of clustering provides consistent results of their qualifications. On the basis of results of this study, it can be concluded that in future study of genetic diversity like here especially in breeding programs of triticale genotypes, more than one marker systems should be used for higher genetic resolution of the genome.

Key words: genetic diversity, triticale, ISSR, RAPD

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