In vitro Antioxidant Activity of Methanol Extract of Allium scorodoprasum

V. D. MITIC, V. P. STANKOV-JOVANOVIC, M. D. ILIC, J. S. CVETKOVIC, M. V. DIMITRIJEVIC and G. S. STOJANOVIC
University of Nis, Faculty of Science and Mathematics, Department of Chemistry, Nis, Serbia

Abstract

MITIC, V. D., V. P. STANKOV-JOVANOVIC, M. D. ILIC, J. S. CVETKOVIC, M. V. DIMITRIJEVIC and G. S. STOJANOVIC, 2014. In vitro antioxidant activity of methanol extract of Allium scorodoprasum. Bulg. J. Agric. Sci., 20: 1130-1136

 

The antioxidant properties of methanol extract of Allium scorodoprasum bulb and seed were evaluated, through determination of total phenolics and flavonoids content, as well as DPPH• (1,1-diphenyl-2-picrylhydrazyl) radical scavenging and ferric thiocyanate methods. Bulb extract showed greater reducing power (AEAC value per 1 mg of dry extract for bulb was 6.00 ±1.03 mg) and ability to scavenge DPPH radical, as well as total phenolic (36.692 ± 4.475 μg GA/mg of dried bulb extract) and flavonoid content. Total phenolic and flavonoid content assay were correlated with ferric reducing power and “scavenging” radical capacity test, and correlation among them was significant. Our research showed that Allium scorodoprasum can be considered as plant with antioxidant compounds.

Key words: Allium scorodoprasum, antioxidant activity, phenolic compounds, correlation
Abbreviations: RO S-reactive oxygen species; SOD- enzymes superoxide dismutase; C-ase- catalase; P-aseperoxidase; GP-ase-glutathione peroxidase; MDA –malonyldialdehide; O•2 - -superoxide; •OH- hydroxyl radicals; GSH -reduced glutathione; DPPH - 2,2-diphenyl-1-picrylhydrazyl; BHT –butylhydroxytoluene; Trolox - 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid; G.A. -gallic acid; A.A. -ascorbic acid; GAE -gallic acid equivalent; RE -rutin equivalent; AEAC –ascorbate equivalent antioxidant capacity

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