Purification and Characterization of Proteases from Streptomyces Strains

Shumen University, Department of Biology, BG – 9700 Shumen, Bulgaria


KALCHEV, K., 2014. Purifi cation and characterization of proteases from Streptomyces strains. Bulg. J. Agric. Sci., Supplement 1: 66–68


Proteolytic enzymes are essential for cell growth, physiology and differentiation in genus Streptomyces. The objective of this work was to study the conditions for production and characteristics of proteases secreted by three newly isolated Streptomycesstrains. The assayed Streptomyces strains produced proteases that degrade casein and beta-lactoglobulin. Proteolytic activity of extracellular proteases was higher on casein and was signifi cantly reduced when the substrate was beta-lactoglobulin. One of the secreted proteases showed specifi c activity against beta-lactoglobulin. This could be perspective for future research as anti-allergic substance.

Key words: Streptomyces, protease, protease activity


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