Effect of Plant Growth Regulators on Chloroplast Ultra Structure in Lamium Album Plantlets

M. STEFANOVA, D. KOLEVA and T. GANEVA
Sofia University “St. Kliment Ohridski”, Department of Botany, Faculty of Biology, BG - 1164 Sofia, Bulgaria

Abstract

STEFANOVA, M., D. KOLEVA and T. GANEVA, 2013. Effect of plant growth regulators on chloroplast ultra structure in Lamium album plantlets. Bulg. J. Agric. Sci., 19: 1208-1212

 

Lamium album L. stem nodal segments were grown on Murashige and Skoog’s medium without growth regulators (control) and on media supplemented with 0.2 mg l^-1 N⁶-benzyladenine, 0.5 mg l^-1 indole-3-butyric acid or both plant growth regulators. The plastid apparatus of in vitro formed leaflets was studied by transmission electron microscopy. The cytokinin N6-benzyladenine negatively affected the chloroplast formation when was applied individually or in combination with the auxin indole-3-butyric acid. On the contrary, IBA in concentration of 0.5 mg l^-1 was appropriate for development of chloroplasts with regular shape and well organized internal membrane system thus providing for the regeneration capacity in vitro.

Key words: in vitro-regeneration, indole-3-butyric acid, medicinal plant, N⁶-benzyladenine, transmission electron microscopy, white dead nettle
Abbreviations: BA - N⁶-benzyladenine; IBA - indole-3-butiric acid; PGRs - plant growth regulators; TE M - transmission electron microscopy

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