New Approaches for in vitro Propagation of Oil-Bearing Rose

AgroBioInstitute, BG - 1164 Sofia, Bulgaria


GINOVA, A. and V. KONDAKOVA, 2013. New approaches for in vitro propagation of oil-bearing rose. Bulg. J. Agric. Sci., 19: 1198-1203


This research was undertaken to overcome two essential problems, which arise during the process of micropropagation of Rosa damascena Mill. f. trigintipetala Dieck. - in vitro rooting and top necrosis. These problems decrease application of in vitro technologies for mass production of oil – bearing rose plants.
The nodal segments from young, active growing shoots were cultured on MS medium with 0.5 mg l-1 BA for shoot induction. Then they are transferred on proliferation medium including QL, 0.5 mg l-1 BA, 0.1 mg l-1 IBA, 0.1 mg l-1 GA3, 0.1 g l-1 Fe-EDD HA. In this study was tested 3 media (MS, QL, WPM) with different combination of hormones for induction of roots. The combination of 1/2 QL macro salt, QL micro salt and vitamins, 20 g l-1 sucrose, 0.4 mg l-1 IBA, 0.4 mg l-1 IAA, 250 mg l-1 meso – inositol and 250 mg l-1 casein hydrolizate (liquid medium) was the most suitable treatment for rooting. The shoots, which developed roots, are 90%. It was used a temporary immersion bioreactor system RITA® for optimize the micropropagation system. It was very effective for overcome the problem with necrosis because factors, which provoke it, were eliminated.
In this article, we report for first time potential capacity of bioreactor system “RITA” for propagation of recalcitrant species as Rosa damascenа Mill.

Key words: Rosa damascena Mill. f. trigintipetala Dieck., in vitro rooting, top necrosis, bioreactors
Abbreviations: MS - Murashige & Skoog Medium, QL - Quirin and Lepoivre Medium, BA - Benzyl Adenine, NAA - 1-Naphthaleneacetic acid, IAA - Indole-3-acetic acid, GA3 - Gibberellic acid, IBA - Indole-3-butyric acid, WPM – Woody Plant Medium, PVP –Polyvinylpyrrolidone, CH – Casein hydrolizate, AC – Active Charcoal

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