Direct Plant Regeneration from Stem Explants of Swainsona salsula Taubert: The Stimulatory Effect of Silver Nitrate and Sucrose on Shoot Induction

G. D. CHEN, J. YANG, F. QIN, H. LIU, S. SHEN, W. Y. CHEN and X. B. MA
College of Life Sciences, China West Normal University, Nanchong, Sichuan 637009, P. R. China

Abstract

CHEN, G. D., J. YANG, F. QIN, H. LIU, S. SHEN, W. Y. CHEN and X. B. MA, 2011. Direct plant regeneration from stem explants of Swainsona salsula Taubert: the stimulatory effect of silver nitrate and sucrose on shoot induction. Bulg. J. Agric. Sci., 17: 339-347

An efficient regeneration system for large-scale propagation of Swainsona salsula Taubert was developed using stem explants. Stem segments were cultured on Murashige and Skoog’s medium containing BA, NA, and silver nitrate in various concentrations, and it was found adventitious shoots regenerated directly from explants on most media with silver nitrate. The best response in terms of frequency of shoot induction (79.62%) and shoot number per explant (6.32) was observed on medium supplemented with 3.0 mg·L^-1 BA, 0 mg·L^-1 NA, and 2.0 mg·L^-1 AgNO₃. Based on the optimal combination of the aforementioned three factors, single factor experiment showed that 60g·L^-1 sucrose was optimum for shoot induction. The rate of shoots induction rose up to 86.67%, and the average shoot number increased from 6.32 to 8.36. After transferred to half strength MS medium, 85.14% shoots rooted easily on medium with 2.0mg·L^-1 IBA. In vitro propagated plants could be transferred to soil with
82.5% survival rate.

Key words: Swainsona salsula Taubert, stem explant, silver nitrate, regeneration, in vitro, sucrose
Abbreviations: BA: 6-benzylaminopurine, NAA: α-naphthalene acetic acid, Suc: sucrose, IBA: Indole-3-butyric acid, GA₃: Gibberellin A₃, MS: Murashige and Skoog (1962) medium, AgNO₃: silver nitrate

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