At. KOTZAMPIGIKIS1, D. HRISTOVA2 and E. TASHEVA-TERZIEVA3
1 Agricultural University, BG -4000 Plovdiv, Bulgaria
2 Plant Protection Institute, BG -2230 Kostinbrod, Bulgaria
3 University of Sofia, Faculty of Biology, BG -1472 Sofia, Bulgaria
KOTZAMPIGIKIS, At., D. HRISTOVA and E. TASHEVA-TERZIEVA, 2010. Virus-vector relationship between Potato leafroll virus PLRV and Myzus persicae Sulzer. Bulg. J. Agric. Sci., 16: 412-421
Experiments were carried out to determine the periods of acquisition, latent period, inoculation and retention of Potato leaf roll virus when transmitted by the green peach aphid. Trials were set up with the indicators Physalis floridana L. and Datura stramonium L., and the method DAS-ELISA was used to determine the latent carriers of the virus. It was established that after acquisition of PLRV, M. persicae remains viruliferous for its lifetime. The optimal period for acquisition of PLRV by the green peach aphid is determined to be 6 hours, and the minimal is 30 minutes. The latent period of the virus in the vector is 6 hours, whereas the optimal period for inoculation of PLRV by the green peach aphid is 120 minutes and the minimal is 30 minutes. The statistical analysis of the obtained results includes one-way analysis of variance (ANOVA) and Student-Newman-Keuls test for multiple comparisons (SNK).