Glutenin Composition of Winter Wheat Varieties Bred in Dobrudzha Agricultural Institute

D. ATANASOVA, N. TSENOV, I. TODOROV and I. IVANOVA
Dobrudzha Agricultural Institute, BG-9520 General Toshevo, Bulgaria

Abstract

ATANASOVA, D., N. TSENOV, I. TODOROV and I. IVANOVA, 2009. Glutenin composition of winter wheat varieties bred in Dobrudzha agricultural institute. Bulg. J. Agric. Sci., 15: 9-19

A total of 70 bread wheat varieties bred in Dobrudzha Agricultural Institute (DAI), Bulgaria were characterized for the composition of high-molecular-weight and low-molecular-weight glutenin subunits (HMW-GS and LMW-GS, respectively) using SDS-PAGE. Twenty-five different HMW-GS patterns were observed. The most frequent combination was 2*, 7+9, 5+10 (23.76%) and N, 7+9, 5+10 (14.85%). Twenty-eight different LMW-GS patterns were established as Glu-A3c, Glu-B3b, Glu-D3c and Glu-A3e, Glu-B3b, Glu-D3c being the most frequent. The average Glu-score of the investigated wheat varieties was comparatively high (8.0). One of the reasons for the high score was the gradually removing of subunit 2+12 at locus Glu-D1, which effect on bread making quality was negative. The potential of locus Glu-D1 was still unused for improving end-use quality of wheat and for broadening the genetic diversity of germplasm. The established database describing the HMW and LMW-GS of DAI bread wheat varieties may be useful for breeders who want to use Bulgarian germplasm in their breeding programs.

Key words: HMW-GS, LMW-GS, bread wheat, DAI varieties
Abbreviations: DAI - Dobrudzha Agricultural Institute, HMW-GS – high-molecular-weight glutenin subunits; LMW-GS - low-molecular-weight glutenin subunits; SDS-PAGE - Sodium Dodecylsulphate PolyAcrylamide Gel Electrophoresis

See the article as a PDF