In vitro Characteristics of Different Pyrenophora Tritici-Repentis Isolates

University of Forestry, Faculty of Agriculture, BG – 1756 Sofia, Bulgaria


ANDONOVA, R. and M. TODOROVA, 2007. In vitro characteristics of different Pyrenophora tritici-repentis isolates. Bulg. J. Agric. Sci., 13: 673-678

Eight ascosporic isolates of different origin were cultivated on PDA, V-8 and Oat agar in controlled conditions (temperature of 22°C±2°C), at photoperiod of 12 h light vs. 12 h darkness, 24 h light vs. 24 h darkness). A morphological characteristics in vitro was performed to identify their radial growth, coloring and compactness of the mycelium formed as well as nutrient medium coloring. Statistically significant differences between the isolates grown on the same nutrient medium and same conditions were found, proving phenotypical and genetic differences between them.

Key words: Pyrenophora tritici-repentis, ascosporic isolates, radial growth, culture characteristics

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