A. I. KRASTANOV1, V. K. GOCHEV2 and T. D. GIROVA2
1 Dep. "Biotechnology", University of Food Technologies, BG - 4000 Plovdiv, Bulgaria
2 Dep. "Biochemistry and Microbiology", "Paisiy Hilendarsky" University of Plovdiv, BG - 4000 Plovdiv, Bulgaria
KRASTANOV, A. I., V. K. GOCHEV and T. D. GIROVA, 2007. Nutritive medium dependent biosynthesis of extracellular laccase from Trichoderma spp. Bulg. J. Agric. Sci.,13: 349-355
The effect of nutritive medium composition on laccase production by Trichoderma viride and Trichoderma longibrachiatum was studied. On the basis of the results obtained it was determined that glucose concentration higher than 20 g/l caused catabolic repression of laccase production The studied phenolic compounds stimulated fungal growth, but only caffeic acid increased laccase production by T. longibrachiatum. The highest stimulating effect demonstrated CuSO4 at concentration 10 mg/ml. Maximum laccase activities 3.00 U/ml for T. viride and 2.25 U/ml for T. longibrachiatum was reached at 96 h of cultivation which correspond to the beginning of stationary phase of strain development.