Effect of Genotype, Explant Type and Culture Medium on Shoot Regeneration in Tomato (Lycopersicon esculentum Mill.) in vitro

1 Maritsa Vegetable Crops Research Institute, BG- 4003 Plovdiv, Bulgaria
2 Institute of Genetics Akad. D. Kostoff, BAS, Sofia, Bulgaria


GROZEVA, S., V. RODEV and Z. DANAILOV, 2006. Effect of genotype, explant type and culture medium on shoot regeneration in tomato (Lycopersicon esculentum Mill.) in vitro. Bulg. J. Agric. Sci, 12: 435-439

The regeneration capacity of three explants types (segments from hypocotyls, cotyledons and true leaves) in eight tomato lines (Lycopersicon esculentum Mill.) was studied. Explants were cultured on MS basal medium supplemented with different concentrations of plant growth regulators BA and IAA. In the studied treatments of these regulators highest regenerative activity was established in leave explants (44.0% and 36.4%, respectively), comparatively lower in the cotyledon (33.3% and 30.6%, respectively) and hypocotyl segments (21.7% and 19.3%, respectively). The highest regenerative frequency was observed in line XXIVA (65.3%) and the lowest one - in line No 8 (3.6%). The best results were obtained in medium supplemented with 2.0 mg/1 BA and 0.2 mg/1 IAA that proves the strongest dependence of in vitro regeneration on genotype as well as less on type of explants and medium composition.

Kay words: Lycopersicon esculentum Mill., tomato, in vitro regeneration habits