V. ATANASOV, Y. STAIKOV and G. NIKOLOV
Thracian University, Agricultural Faculty, BG-6000 Stara Zagora, Bulgaria
ATANASOV, V., Y. STAIKOV and G. NIKOLOV, 2006. Sperm diluent VKA-3 for artificial propagation of carps (Cyprinus carpio L.). Bulg. J. Agric. Sci.,12: 176-184
Experiments were carried out with several world known sperm activating media for artificial insemination of eggs from freshwater fishes. It was found that extender VKA-3 increasing the ejaculate volume, does not activate the Cyprinus carpio L. spermatozoa and does not have negative effect on the gametes. VKA-3 protects carp spermatozoa from destructive changes of the membranes - fact marked by low cholesterol level in seminal plasma. Medium VKA-2 have the best sperm activating effect and may use along with diluent VKA-3 for artificial insemination of carp's eggs and multiply of genetic-esteemed fish males. The obtained reproductive effect was more 22% by using of sperm activator VKA-2 - single or along with extender VKA-3. The application of that media will be optimized the carp's propagation processes.