Influence of the Genotype on the Development of Isolated in vitro Embryos of Sugar and Fodder Beet Crosses

Agricultural Institute, BG-9700 Shumen, Bulgaria


KIKINDONOV, Tz., 2003. Influence of the genotype on the development of isolated in vitro embryos of sugar and fodder beet crosses. Bulg. J. Agric. Sci., 9: 343–348

It has been studied the influence of the parental genotypes on the development of isolated in vitro immature embryos of crosses of two MS sugar beet lines with 7 diploid and 7 tetraploid fodder beet pollinators. A weak correlation was established between the in vitro development of unpollinated ovules and immature embryos for the diploid forms (r = 0.186), in comparison with that for the fodder beet tetraploids (r = 0.444). The development of the hybrid embryos is determined at greater extend from the female genotype, manifested in the in vitro gynogenesis. The correlation between the development of unpollinated ovules and immature embryos from crosses of MS plants with diploid pollinators is r = 0.685, and with the tetraploid pollinators is r = 605. The low level of variation (CV = 38.4 %) for developed hybrid embryos and the correlation with the gynogenesis in the pollinators (r = 0.125) is an evidence of the weak influence of the paternal genotype. The higher frequency of development in the crosses with tetraploid pollinators is considered as a specific manifestation of the heterosis effect of the triploid sugar beet hybrids.

Key words: fodder beet, sugar beet, hybrids, in vitro gynogenesis, ovules, embryos.
Abbreviations: MS lines – male-sterile lines