Inhibition of Enteropathogenes by Lactic Acid Bacteria, Isolated from Healthy Suckling Children

Trakia University, Agrarian Faculty, BG-6000 Stara Zagora, Bulgaria


BOYCHEVA, S., 2003. Inhibition of enteropathogenes by lactic acid bacteria, isolated from healthy suckling children. Bulg. J. Agric. Sci., 9: 95–100

36 strains of lactic acid bacteria (LAB) – 21 rods and 15 cocci, isolated from feces of healthy, naturally fed children from 4 to 30 days old were investigated. The isolated rods were determined as lactobacilli – 15 of them as obligate heterofermentative, 4 – as facultative heterofermentative and 2 – as obligate homofermentative. The isolated cocci were identified as enterococci.
Inhibitori activity (IA) of the isolated strains of LAB against 3 strains of enteropathogenic E. coli and Salmonella enteritides, Salmonella typhimurium, Salmonella gallinarum was examinated. 6 of the isolated lactobacilli and 5 of isolated enterococci showed over 50% IA against used test organisms. They were identificated as L. plantarum, L. reuteri, L. brevis, E. faecalis and E. faecium. These strains will be used for preparation of probiotics for regulation of human intestinal microflora.

Key words: lactobacilli, enterococci, inhibitory activity, enteropathogenes, children