Efficient Plant Regeneration in Culture from Immature Inflorescences in Durum Wheat Cv. Progress

Cotton and Durum Wheat Research Institute, BG-6200 Chirpan, Bulgaria


BOZHANOVA, V., 2003. Efficient plant regeneration in culture from immature inflorescences in durum wheat cv. Progress. Bulg. J. Agric. Sci., 9: 209–213

Immature inflorescences grouped into 3 groups depending on their size of three durum wheat genotypes were cultivated on MS medium supplemented with 2 mg/l 2.4 D and 0.5 mg/l ABA. After 3–4 weeks embryogenic and non embryogenic types of calli were observed depending on the genotype and the explants size. After calli transferring onto regenerating medium with 0.5 mg/l NAA and 1.0 mg/l kinetin the most plants were regenerated in cv. Progress (433%) from explants with length from 5 to 10 mm. Results of the analysis of variance showed that for both studied traits (callus induction and plant regeneration) genotype, size of explants and interaction were highly significant. In the second experiment with field-grown donor plants the high regeneration potential of cv. Progress and the reliability of the protocol used were confirmed.

Key words: durum wheat, in vitro cultivation, immature inflorescences, explants size, donor plants growth conditions, plant regeneration