Growth and Leaf Gas Exchange of in vitro Produced Grapevine Plants Grown in Different Substrates

Agricultural University BG-4000 Plovdiv, Bulgaria


PANDELIEV, S., Z. ZLATEV and M. DIMKU, 2002. Growth and leaf gas exchange of in vitro produced grapevine plants grown in different substrates. Bulg. J. Agric. Sci., 8: 1–6

In the period 1997–1999, a study was conducted on the growth and leaf gas exchange of grapevine plants in the process of adaptation to new, homogeneous and nutrient-balanced substrates. The investigations were carried out with the rootstock Fercal and the grape cultivar Cabernet Sauvignon. It was established that the plants of variants V3 (soil – 5%, sand – 5%, flax shavings – 15%, farmyard manure – 5%, rice husks – 20%, and a compost from grapevine canes, grape marc and rachises – 50%) and V4 (sand – 5%, peat – 20%, flax shavings – 50%, farmyard manure – 5%, and rice husks – 20%) showed the highest photosynthetic and transpiration rates and hence increasing the photosynthetic water use efficiency. The highest leaf content of photosynthetic pigments was also established in these variants. The highest growth was observed in the plants of variants V4, V3, and V2 (soil – 20%, sand – 5%, rice husks – 20%, flax shavings – 20%, and compovet B-4 – 35%), which was due to the better leaf structure and the high functional activity of the photosynthetic apparatus.

Key words: grapevine, tissue culture, substrate, growth, leaf gas exchange, chlorophyll