Differences in Pathogenicity between Some Bulgarian Bean Common Mosaic Virus (BCMV) Isolates and a Standard Range of BCMV Pathotypes
S. TSORLIANIS1 and D. KOSTOVA2
1 Agricultural University, BG-4000 Plovdiv, Bulgaria
2 Maritsa Vegetable Crops Research Institute, BG-4003 Plovdiv, Bulgaria
TSORLIANIS, S. and D. KOSTOVA, 2000. Differences in pathogenicity between some Bulgarian Bean Common Mosaic Virus (BCMV) isolates and a standard range of BCMV pathotypes. Bulg. J. Agric. Sci., 6: 295-302
BCMV (Bean common mosaic virus)/ BCMNV (Bean common mosaic necrotic virus) isolates collected from different bean growing regions in Bulgaria were identified based on serology and biological assay on Chenopodium quinoa. Double Antibody Sandwich (DAS) and Plate Trapped Antibody (PTA) ELISA were carried out using one monoclonal and two polyclonal antibodies. Seventeen BCMV/BCMNV isolates in pure infection were characterized according to the phenotypic expression they induced on a standard range of differential varieties. Most of isolates were proved to belong to temperature dependent necrotic strains of BCMV. These isolates expressed pathogenicity phenotypes significantly different from those of the standard ones and therefore were considered as novel. Two isolates were classified as BCMNV similar to pathotype VIa.
Key words: BCMV, BCMNV, beans, Bulgaria, serology, characterization, virus strains, temperature dependent necrotic strains, differential varieties