Vitality and Acrosomal Integrity of Buffalo Spermatozoa Frozen in Straws Using Different Rates of Thawing


Buffalo Research Institute with Biotechnology Centre, BG-9700 Shoumen, Bulgaria


KOLEV, S. I., 2000. Vitality and acrosomal integrity of buffalo spermatozoa frozen in straws using different rates of thawing. Bulg. J. Agric. Sci., 6: 477-480

The aim of the study was to determine the effect of different regimes of thawing on motility and acrosomal integrity of buffalo spermatozoa frozen in straws. Fifteen ejaculates from mature buffalo bulls Bulgarian Murrah breed were tested. Semen was extended in PG20 extender containing as exocryoprotector high molecular polyethylen glycol (kD 20000) and packaged in straws. After freesing and one year storage in liquid nitrogen at 196 0C semen was thawed at water bath temperatures of 30, 38 and 75 0C for motility and acrosomal integrity. The thawing of spermatozoa at 380C for 30 s resulted in higher % motility and intact acrosomes of spermatozoa while regime 38 0C for 15 s was found to be the next in order of merit. Reduction of motility and percentage of intact acrosomes was most evident when spermatozoa were thawed at 750C for 9 s.

Key words : spermatozoa, buffalo, thawing rate