Screening Tests of Trypsin Protease Inhibitor with Respect to Some Phytopathogenic Fungi


Agricultural University, Department of Agroecology, BG-4000 Plovdiv, Bulgaria


NIKOLOV, A., 2000. Screening tests of trypsin rrotease inhibitor with respect to some phytopathogenic fungi. Bulg. J. Agric. Sci., 6: 257-259

Paprika seeds (Capsicum annium) is real source of proteinase inhibitors of peptide type. The suppressive action of this proteinase vas preliminary tested in laboratory as well as under greenhouse conditions using the phytopathogenic fungus as follows: Venturia inaequalis, Phragmidium mucronatum, Fusarium culmorum, Alternaria solani. The inhibitor was applied solely in 0.05 to 3% as well as in combination with synthetic fungicides:Sportac 40 EC (prohloraz) and Score 250 EC (difenconazol) in their optimal concentrations, half, five and ten time lower. Inhibitors solutions 0.5-2.% showed 67-95% suppression of Venturia inaequalis and 75-95% effectiveness in relation to Phragmidium mucronatum. According to other studied phytopathogenes the biological activity was rather lower but 68% suppresion of Alternaria solani was observed using 3% inhibitor solution. The combinations of proteinase inhibitor by fungicides Sportak 40 EC and Score 250 EC (in optimal or half concentrations) improved significantly the suppression of Alternaria solani and Fusarium culmorum.

Key words: tryspin protease inhibitor, fungi, phytopathogenic