A comparative study on raspberry cultivars in micropropagation

Maria Georgieva1, Violeta Kondakova2 and Svetla Yancheva3
1 Agricultural Academy, Research Institute of Mountain Stockbreeding and Agriculture, 5600 Troyan, Bulgaria
2 Agricultural Academy, Agrobioinstitute, 1164 Sofia, Bulgaria
3 Agricultural University, 4000 Plovdiv, Bulgaria

Abstract

Georgieva, M., Kondakova, V. & Yancheva, S. (2020). A comparative study on raspberry cultivars in micropropagation. Bulg. J. Agric. Sci., 26 (3), 527–532

The aim of the present study was to clarify the effectiveness of a simplified protocol for micropropagation of raspberry cultivars, such as Samodiva (control), Meeker, Willamette and the candidate cultivar of Magdalena (passed through DUS test) as prospective ones, which are suitable for cultivation in the mountain and hilly regions of Bulgaria. The effect of growth regulators BAP (0.5 mg l-1) and IBA (0.01 mg l-1) on proliferation capacity and shoot length over six passages was established. The highest multiplication potential 3.9 at the fifth passage and average length of the shoots 3.76 cm (fourth passage) were registered in the candidate cultivar of Magdalena. The best rhizogenic ability was recorded in Samodiva – 80.5%. These results show that genotype is the primary factor determining a high propagation and economically significant efficiency in this process. The application of simple medium for all studied genotypes is towards commercial propagation, comparing their growth characteristics and obtaining pre-base material for planting in a production nursery and further investigations.

Keywords: in vitro propagation; raspberries; growth regulators; proliferation capacity; root formation
Abbreviations: BAP – benzylaminopurine; IBA – indole-3-butyric acid; GA3 – gibberellic acid; MS – basic nutrient media (Murashige&Skoog,1962)

See the article as a PDF.