Early events during the induction of somatic embryogenesis in genera Medicago

Anelia Iantcheva, Miglena Revalska
AgroBioInstitute, Blvd. Dragan Tzankov 8, 1164 Sofia, Bulgaria

Abstract

Iantcheva. A. & Revalska, M. (2018). Early events during the induction of somatic embryogenesis in genera Medicago. Bulgarian Journal of Agricultural Science, 24(6), 1042–1052

In this review based on our data obtained in last 25 years we outline the early events of the induction of somatic embryogenesis in genera Medicago and in the model species - diploid M. truncatula andtetraploid M. falcata. Following the collected data considerable attention is paid on the factors affecting the process of the induction, activation of somatic cells for division by using an auxin 2,4-D (2,4-Dichlorophenoxyacetic acid) as a trigger, type of first cell division and further development. Based on recently established results the role of auxin in somatic embryo development is traced and confirmed by the localization of expression of the genes encoding an auxin influx carrier (MtLAX3) and the transcriptional factor an auxin response factor B3 (MtARF-B3) from the genome of M. truncatula. A brief consideration is paid to the role of genotype, explant pre-treatment and genome size.

Keywords: somatic embryogenesis; Medicago truncatula; Medicago falcata; asymmetric division

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