Isolation and characterization of biosurfactant-producing bacteria isolated from agriculture area in Thailand

Pannapa Powthong1, and Pattra Suntornthiticharoen2
1 Faculty of Medical Technology, Rangsit University, Pathumthanee, Thailand 12000
2 Department of Medical Sciences, Faculty of Science, Rangsit University, Pathumthanee, Thailand 12000

Аbstract

Powthong, P., & Suntornthiticharoen, P. (2018). Isolation and characterization of biosurfactant-producing bacteria isolated from agriculture area in Thailand. Bulgarian Journal of Agricultural Science, 24(4), 623–630

Biosurfactant-producing bacteria were isolated from various soil and water in agricultural area of Thailand. Based on hemolytic activity, drop collapsing test, oil displacement test, as well as emulsification activity (%EA), 8 effective isolates from 234 isolates were selected. All selected isolates were tested for antimicrobial activities against plant pathogenic bacteria. It was revealed that all isolates can inhibit more than 2 tested-plant pathogenic bacteria. The crude extracts of selected isolates were tested for stability of pH, NaCl, CaCl2, MgCl2 and temperature by measuring %EA and showed stability over a broad range of these conditions. All interested isolates were identified by biochemical test and MALDI-TOF MS. The isolates were: 1 isolate Alcaligenes faecalis subsp. faecalis, 2 isolates Proteus mirabilis, and 1 isolate Providencia alcalifaciens. Overall, the strains found in this study were useful for the bioremediation of the agricultural area.

Keywords: biosurfactant; agricultural area; biodegradation; antimicrobial activity

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