HAYET BELTAYEF1,2; MONGI MELKI2; WAFA SAIDI2; SARRA SAMAALI3; ADELE MUSCOLO4; CRISTINA CRUZ5; TAWFIK GAROUI2
1 University of Carthage, Faculty of Sciences of Bizerte, Box 77 - 1054 Amilcar, Tunisia
2 University of Jendouba, Higher School of Agriculture of Kef, 7119 Le Kef, Tunisia
3 University of Sousse, High Agronomic Institute of Chott Mariem, B.P. 47 - 4042 Chott Meriem - Sousse, Tunisia
4 Mediterranea University of Reggio Calabria, Salita Melissari, 89124 Reggio Calabria, CF 80006510806, Italy
5 Universidade Lisboa, Centre for Ecology, Evolution and Environmental Changes, Faculdade de Ciências Campo Grande 1749-016 Lisboa, Portugal
Beltayef, H., M. Melki, W. Saidi, S. Samaali, A. Muscolo, C. Cruz and T. Garoui, 2018. Betterment of biological nitrogen fixation in snap bean under Mediterranean semi-arid conditions. Bulg. J. Agric. Sci., 24 (2): 244–251
A greenhouse experiment was implemented to evaluate the influence of Rhizobia strain inoculation, on growth, nitrogen purport, nodulation and enzymatic activities of common bean (Phaseolus vulgaris L.). The factors studied are two varieties of beans and three strains of Rhizobium. The results indicate that the two indigenous strains are low effective than the international strain (tropici CIAT 899), which present the best results for all parameters analyzed whatever the variety. A significant interaction was detected between varieties and rhizobia for the number and dry weight nodule (p ≤ 0.05). In fact, Plants inoculated with R. tropici CIAT 899 showed the highest total biomass by fixing the most atmospheric nitrogen.
The enzyme test linked to the nitrogen assimilation process was realized in leaves of bean. It was revealed that the activities of GOGAT and GS were higher than GDH and MDH activities. R. Ciat 899 inoculation showed the up mostest values in all enzymatic activity compared to the control and native Rhizobium. However, our study pointed out the high affinity between Rhizobium tropici and the Italian variety “Garrafel Enana”.