Agrobacterium Rhizogenes – Mediated Hairy Root Induction in Garlic

FATEMEH MORADI; MAHBOOBEH ZARE MEHRJERDI; KOUROSH VAHDATI
University of Tehran, College of Aburaihan, Department of Horticulture, 14155-6619 Tehran, Iran

Abstract

Moradi, F., M. Z. Mehrjerdi and K. Vahdati, 2017. Agrobacterium rhizogenes – mediated hairy root induction in garlic. Bulg. J. Agric. Sci., 23 (4): 527–577

Three factors including Agrobacterium rhizogenes strains (A4, A7 and ATCC15834), genotypes (Gorgan and Ramhormoz) and explant types (stem disc meristem, shoot apical meristem and root apical meristem) were conducted to study the effects on hairy root induction of garlic. Our results showed that A. rhizogenes strains and explants types had signifi cant effect on root induction frequency (RIF), while the genotypes hadn’t effect on the RIF. The frequency of root induction was up to 23.3%. Some the combination of three factors could induce hairy roots.The results of PCR analysis showed that expected fragments with length of 780bp corresponding to the rolB gene was amplifi ed from hairy root cultures. To our knowledge, this study was the first comprehensive evaluation of garlic hairy root induction.

Key words: Agrobacterium rhizogenes; explants; garlic; genotype; hairy root

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