Development of ISSR Markers for a Bulgarian Tomato Breeding Collection Aiming to Improve Antioxidant Compounds in Fruits

ANGELOV MICHAIL1; BILYANA IVANOVA1; ATANAS PAVLOV2; DANIELA GANEVA3; ZHIVKO DANAILOV4; BOJIN BOJINOV1
1 Agrucultural University of Plovdiv, Department of Genetics and Plant Breeding, BG-4000 Plovdiv
2 Bulgarian Academy of Science, The Stephan Angeloff Institute of Microbiology, Laboratory of Applied Biotechnologies, BG-4000 Plovdiv, Bulgaria
3 Agrucultural Academy, Maritsa Vegetable Crops Research Institute, BG-4000 Plovdiv, Bulgaria
4 Bulgarian Academy of Science, Institute of Plant Physiology and Genetics, Bldg. 21, BG-1113 Sofi a, Bulgaria

Abstract

Angelov, M., B. Ivanova, A. Pavlov, D. Ganeva, Zh. P. Danailov and B. M. Bojinov, 2017, Development of ISSR markers for a Bulgarian tomato breeding collection aiming to improve antioxidant compounds in fruits. Bulg. J. Agric. Sci., 23 (3): 405–410

The Inter-Simple Sequence Repeat marker technique was applied to a set of tomato genotypes that were found to diverge in antioxidant compounds in fruits. As marker-assisted selection is becoming a tool that brings major advantages for breeding programs need is rising to use it to quickly respond to consumer demand. Being part of a system for effi cient plant breeding in many crops the aim of the present study was to test Inter-Simple Sequence Repeats as candidate tool for incorporating in a breeding program aiming to improve antioxidant properties of tomato fruits. Application of Inter-Simple Sequence Repeat markers resulted in obtaining adequate representation of every accession from the selected group. This opens the opportunity for using the system in confi rming the hybrid nature of the F1 progenies and further accelerating the breeding of genotypes for use as healthy food.

Key words: Solanum lycopersicum; molecular markers; Inter-Simple Sequence Repeats; ISSRs

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