In vitro Propagation of the Balkan Endemic Species Verbascum eriophorum Godr.

Zh. P. YORDANOVA, M. A. ROGOVA and V. M. KAPCHINA-TOTEVA
Sofi a University “St. Kliment Ohridski”, Department of Plant Physiology, Faculty of Biology, BG-1164 Sofia, Bulgaria

Abstract

YORDANOVA, Zh. P., M. A. ROGOVA and V. M. KAPCHINA-TOTEVA, 2016. In vitro propagation of the Balkan endemic species Verbascum eriophorum Godr. Bulg. J. Agric. Sci., 22: 767–771

In vitro shoot culture of the Balkan endemic species Verbascum eriophorum Godr. was induced by successful sterilization
of seeds with 70% ethanol. Regenerated plants on hormone free MS medium had low growth index, shortened internodes and well developed root system. Then the effect of different concentrations of cytokinin BA (6-benzylaminopurine) and auxin IBA (indole-3-butyric acid) on the in vitro multiplication of V. eriophorum was examined. All tested concentrations of IBA (0.1; 0.5; 1.0 mg/L) stimulated root formation but suppressed shoot development and leaf formation. More effective in promoting shoot development was MS medium supplemented with 0.5 mg/L BA and approximately 98% of explants showed shoot proliferation and produced 14.22±0.2 shoots per explant. Subsequently shoots were transferred on hormone free basal MS medium for root development and accumulation of leaf biomass. A collection from in vitro tissue cultures, which is an approach for preservation of V. eriophorum has been established.

Key words: mullein, shoot culture, in vitro multiplication, auxin, cytokinin

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