I. DIMITROVA1, M. BOZHILOVA-SAKOVA1, N. STANCHEVA2 and T. TZONEV3
1 University of Forestry, Agronomy Faculty, BG-1756 Sofia, Bulgaria
2 Agricultural Academy, Agricultural Institute, BG-9700 Shumen, Bulgaria
3 Agricultural Academy, Agricultural Experimental Station, Targovishte BG-7700, 91 Bulgaria
DIMITROVA, I., M. BOZHILOVA-SAKOVA, N. STANCHEVA and T. TZONEV, 2016. Molecular analysis of ovine myostatin gene (MSTN) in Northeast Bulgarian Merino Sheep Breed using PCR-RFLP Method. Bulg. J. Agric. Sci., 22: 315–317
The purpose of this study was to identify genotypes of myostatin gene by PCR-RFLP method in 32 rams from Northeast Bulgarian Merino Sheep Breed. The animals are raised in Agricultural Experimental Station in Targovishte – Bulgaria. Genomic DNA was extracted from blood samples. The polymerase chain reaction was used to amplify 337 bp fragment of exon III of MSTN locus. The PCR products were digested by restriction enzyme HaeIII and the obtained patterns were visualized on 2% agarose gel stained with GelRed. All thirty two animals were monomorphic for the allele m and only genotype mm, respectively.